Knowledge is one of the key factors for company?s success: Companies have to know which knowledge they have, who it has and how it is used within the company to protect and develop their company´s knowledge. Knowledge may be modeled within knowledge structure maps. This paper describes a procedure of how knowledge structure maps based on Multiple Domain Matrices (MDMs) can be created for companies. MDMs are used for analyzing complex systems based on their structural characteristics. The knowledge maps contain the domains knowledge, tasks and employees and their relations to each other. The procedure is evaluated based on a use case within an SME in the mechanical engineering sector.